Hotspot

trnG-UCC-trnR-UCU

LSC · 10054-10228 · 175 bp

Back to Thunbergia

Pi 0.0613
MarkerSeek score 74.80
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
175
variable_sites
10
pi
0.0613
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 243-255 bp 1.0 70.4
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 246-258 bp 1.0 70.1
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 249-261 bp 1.0 69.6
trnG-UCC-trnR-UCU_p4 GACGTTCAAAGCGCTGAATC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.1 56.9 282-294 bp 1.0 68.7
trnG-UCC-trnR-UCU_p5 GTTCAAAGCGCTGAATCGAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.1 56.9 279-291 bp 1.0 68.7