Hotspot

rrn5-rrn4.5

Genome · 59338-59656 · 319 bp

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Pi 0.0334
MarkerSeek score 66.00
Primer available yes
Label rrn5-rrn4.5

Feature Metadata

label_name
rrn5-rrn4.5
feature_type
igs
region
Genome
length_bp
319
variable_sites
16
pi
0.0334
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-rrn4.5_p1 CGAGATGGATTGGTGTGGTT CCACCTCAAGATGAGTGCTC 50.0 55.0 57.9 58.0 483-657 bp 1.0 81.3
rrn5-rrn4.5_p2 GATTGGTGTGGTTCCCCTAC CCACCTCAAGATGAGTGCTC 55.0 55.0 57.9 58.0 476-650 bp 1.0 81.2
rrn5-rrn4.5_p3 CGAGATGGATTGGTGTGGTT AGACGAGCCGTTAGGTGATA 50.0 50.0 57.9 58.0 448-622 bp 1.0 81.2
rrn5-rrn4.5_p4 GATTGGTGTGGTTCCCCTAC AGACGAGCCGTTAGGTGATA 55.0 50.0 57.9 58.0 441-615 bp 1.0 81.2
rrn5-rrn4.5_p5 AACCACCAAGTTCGAGATGG CCACCTCAAGATGAGTGCTC 50.0 55.0 57.8 58.0 495-669 bp 1.0 80.7