Hotspot

atpI-atpH

Genome · 37635-38230 · 596 bp

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Pi 0.0135
MarkerSeek score 67.70
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
Genome
length_bp
596
variable_sites
12
pi
0.0135
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 CGAATCCATGGAGGGTCATC CCTACGGATAATCCAGCAGC 55.0 55.0 57.8 57.9 674-759 bp 1.0 79.5
atpI-atpH_p2 CGAATCCATGGAGGGTCATC GCCTACGGATAATCCAGCAG 55.0 55.0 57.8 57.9 675-760 bp 1.0 79.5
atpI-atpH_p3 CGAATCCATGGAGGGTCATC AACGGAAGCAGCAGAAATCA 55.0 45.0 57.8 58.1 651-736 bp 1.0 79.5
atpI-atpH_p4 CGAATCCATGGAGGGTCATC ACGGAAGCAGCAGAAATCAA 55.0 45.0 57.8 58.1 650-735 bp 1.0 79.5
atpI-atpH_p5 CGAATCCATGGAGGGTCATC GCCAGGTCCAATAGAAGCAA 55.0 50.0 57.8 57.9 696-781 bp 1.0 79.2