Hotspot

trnK-UUU-chlB

Genome · 3877-5892 · 2016 bp

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Pi 0.0988
MarkerSeek score 62.80
Primer available yes
Label trnK-UUU-chlB

Feature Metadata

label_name
trnK-UUU-chlB
feature_type
igs
region
Genome
length_bp
2016
variable_sites
26
pi
0.0988
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.10
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-chlB_p1 AACGACATGCTGCTTTTTCC CTCACATCGAACCATCTGCA 45.0 50.0 57.6 58.0 2606 bp 0.333333 49.8
trnK-UUU-chlB_p2 AACGACATGCTGCTTTTTCC CGAGAAATGGGGATTCGTGT 45.0 50.0 57.6 58.0 2742 bp 0.333333 49.8
trnK-UUU-chlB_p3 TGAGGAACAGAAGCTCGTTC CTCACATCGAACCATCTGCA 50.0 50.0 57.6 58.0 2858 bp 0.333333 49.7
trnK-UUU-chlB_p4 GATCGCTCTCCTGACTCATG TTTGCTCTGGGTATGGAGGA 55.0 50.0 57.6 58.0 2868 bp 0.333333 49.7
trnK-UUU-chlB_p5 AACGACATGCTGCTTTTTCC TTTGCTCTGGGTATGGAGGA 45.0 50.0 57.6 58.0 2427 bp 0.333333 49.7