Hotspot

ndhD

SSC · 114151-114580 · 430 bp

Back to Thrixspermum

Pi 0.221

MarkerSeek score 80.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 430
variable_sites: 60
pi: 0.221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGCACAACGACGAATGAAGA	TCTTTACCGACAAAAGCCCG	45.0	50.0	58.1	58.5	626 bp	0.5	55.0
ndhD_p2	TGCACAACGACGAATGAAGA	TTTACCGACAAAAGCCCGTA	45.0	45.0	58.1	57.5	624 bp	0.5	54.6
ndhD_p3	TGCACAACGACGAATGAAGA	ACCGACAAAAGCCCGTATTC	45.0	50.0	58.1	58.6	621 bp	0.5	54.4
ndhD_p4	TGCACAACGACGAATGAAGA	CGACAAAAGCCCGTATTCGA	45.0	50.0	58.1	58.6	619 bp	0.5	53.7
ndhD_p5	TGCACAACGACGAATGAAGA	ACAAAAGCCCGTATTCGAGA	45.0	45.0	58.1	57.2	617 bp	0.5	52.8