Hotspot

psaC-ndhE

SSC · 114964-115477 · 514 bp

Back to Thrixspermum

Pi 0.0795

MarkerSeek score 78.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 514
variable_sites: 38
pi: 0.0795
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	GTTTCGTCGATCCCTCAACA	50.0	50.0	58.0	57.9	761 bp	0.5	59.2
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	TGTTTCGTCGATCCCTCAAC	50.0	50.0	58.0	57.9	762 bp	0.5	59.2
psaC-ndhE_p3	AGCTCGAACACATTGGGTAC	GTTTCGTCGATCCCTCAACA	50.0	50.0	57.9	57.9	645 bp	0.5	58.6
psaC-ndhE_p4	AGCTCGAACACATTGGGTAC	TGTTTCGTCGATCCCTCAAC	50.0	50.0	57.9	57.9	646 bp	0.5	58.6
psaC-ndhE_p5	GCTCGAACACATTGGGTACA	GTTTCGTCGATCCCTCAACA	50.0	50.0	58.2	57.9	644 bp	0.5	57.8