Hotspot

ycf1-rpl32

SSC · 111367-111814 · 448 bp

Back to Thrixspermum

Pi 0.31

MarkerSeek score 78.40

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 448
variable_sites: 135
pi: 0.31
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	GCTTCCTTCGTAACTCCAGG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	58.0	58.0	919-933 bp	1.0	91.3
ycf1-rpl32_p2	GCTTCCTTCGTAACTCCAGG	TTCGTTCTCAGTCGGAGTCT	55.0	50.0	58.0	58.1	1341-1361 bp	1.0	91.1
ycf1-rpl32_p3	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1110-1124 bp	1.0	90.1
ycf1-rpl32_p4	GCTTCCTTCGTAACTCCAGG	TGATTTTGAGACCCAAGGCC	55.0	50.0	58.0	58.1	1318 bp	0.5	59.3
ycf1-rpl32_p5	GGGGGTTAAGGAGGTTTGTG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	58.1	58.0	668 bp	0.5	59.0