Hotspot

petA-psbJ

LSC · 63728-64761 · 1034 bp

Back to Thrixspermum

Pi 0.0496
MarkerSeek score 77.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1034
variable_sites
49
pi
0.0496
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTGATTTTGGCAC CCGGATTGGGTTCATCTCTG 50.0 55.0 58.0 58.0 1142-1210 bp 1.0 86.2
petA-psbJ_p2 CCATCAAGCTCGATCAACCA CCGGATTGGGTTCATCTCTG 50.0 55.0 58.0 58.0 1257-1325 bp 1.0 85.5
petA-psbJ_p3 GGCATCTGTGATTTTGGCAC GAGAACCGACAAGGGATTCC 50.0 55.0 58.0 58.0 1713-1773 bp 1.0 84.8
petA-psbJ_p4 GGCATCTGTGATTTTGGCAC TCCCTATTCATTGCGGGTTG 50.0 50.0 58.0 57.9 1800-1860 bp 1.0 84.4
petA-psbJ_p5 CCATCAAGCTCGATCAACCA GAGAACCGACAAGGGATTCC 50.0 55.0 58.0 58.0 1828-1888 bp 1.0 84.3