Hotspot

atpB-rbcL

LSC · 54405-55402 · 998 bp

Back to Thrixspermum

Pi 0.0499

MarkerSeek score 83.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 45
pi: 0.0499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGCCCTTGGTTTTGTTCTTC	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.9	58.1	1064-1098 bp	1.0	85.3
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1086-1120 bp	1.0	85.2
atpB-rbcL_p3	TACGCCCTTGGTTTTGTTCT	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.6	58.1	1066-1100 bp	1.0	83.6
atpB-rbcL_p4	CGCCCTTGGTTTTGTTCTTC	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.9	58.4	1065-1099 bp	1.0	82.9
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	1087-1121 bp	1.0	82.8