Hotspot

trnR-UCU-atpA

LSC · 10503-10691 · 189 bp

Back to Thrixspermum

Pi 0.143

MarkerSeek score 84.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 25
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAAAGGCGTCCATTGTCT	CCGAAGAAGCTGAAACCCTT	45.0	50.0	57.5	58.1	345-356 bp	1.0	84.1
trnR-UCU-atpA_p2	AGGAATGAAAGGCGTCCATT	CCGAAGAAGCTGAAACCCTT	45.0	50.0	57.5	58.1	349-360 bp	1.0	83.9
trnR-UCU-atpA_p3	ATGAAAGGCGTCCATTGTCT	TCACCGAAGAAGCTGAAACC	45.0	50.0	57.5	58.1	348-359 bp	1.0	83.9
trnR-UCU-atpA_p4	AGGAATGAAAGGCGTCCATT	TCACCGAAGAAGCTGAAACC	45.0	50.0	57.5	58.1	352-363 bp	1.0	83.6
trnR-UCU-atpA_p5	GGAATGAAAGGCGTCCATTG	CCGAAGAAGCTGAAACCCTT	50.0	50.0	57.4	58.1	348-359 bp	1.0	83.5