Hotspot

rbcL-accD

LSC · 59768-60339 · 572 bp

Back to Thomsonaria

Pi 0.00136

MarkerSeek score 54.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 572
variable_sites: 2
pi: 0.00136
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGGAGTCCGGAACTAGCT	CTTGCCCCTCATTTCCATGA	50.0	50.0	58.1	57.9	689-712 bp	1.0	78.9
rbcL-accD_p2	AATGGAGTCCGGAACTAGCT	TCTTGCCCCTCATTTCCATG	50.0	50.0	58.1	57.9	690-713 bp	1.0	78.9
rbcL-accD_p3	AATGGAGTCCGGAACTAGCT	CGAATCGAACCGCCATTTTT	50.0	45.0	58.1	57.7	721-744 bp	1.0	78.0
rbcL-accD_p4	AATGGAGTCCGGAACTAGCT	ATCGAATCGAACCGCCATTT	50.0	45.0	58.1	58.3	723-746 bp	1.0	77.3
rbcL-accD_p5	AATGGAGTCCGGAACTAGCT	TCGAACCGCCATTTTTCCAT	50.0	45.0	58.1	58.5	717-740 bp	1.0	76.4