Hotspot

trnH-GUG-psbA

LSC · 133-411 · 279 bp

Back to Thomsonaria

Pi 0.0141

MarkerSeek score 49.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 7
pi: 0.0141
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	435-456 bp	1.0	84.4
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	385-406 bp	1.0	84.3
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	388-409 bp	1.0	84.2
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	374-395 bp	1.0	84.2
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	388-409 bp	1.0	84.1