Hotspot

ndhF-rpl32

SSC · 112075-112894 · 820 bp

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Pi 0.0745

MarkerSeek score 79.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 820
variable_sites: 54
pi: 0.0745
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAAAAAGGA	CCAATACCCCTTCCGTTTCC	45.0	55.0	58.0	58.2	997 bp	0.5	57.7
ndhF-rpl32_p2	TGTTGCTGTCGGAAAAAGGA	CGATGTCCAATACCCCTTCC	45.0	55.0	58.0	57.7	1003 bp	0.5	57.6
ndhF-rpl32_p3	TGTTGCTGTCGGAAAAAGGA	GATGTCCAATACCCCTTCCG	45.0	55.0	58.0	57.7	1002 bp	0.5	57.6
ndhF-rpl32_p4	TGTTGCTGTCGGAAAAAGGA	TTGCCTAAGTCGCTCAAGAG	45.0	50.0	58.0	57.6	1585 bp	0.5	56.7
ndhF-rpl32_p5	TGTTGCTGTCGGAAAAAGGA	ACCCCTTCCGTTTCCAAATT	45.0	45.0	58.0	57.6	992 bp	0.5	56.5