Hotspot

trnT-TGT-trnF-GAA

LSC · 45978-46662 · 685 bp

Back to Thlaspi

Pi 0.09

MarkerSeek score 78.90

Primer available yes

Label trnT-TGT-trnF-GAA

Feature Metadata

label_name: trnT-TGT-trnF-GAA
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 54
pi: 0.09
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnF-GAA_p1	CGGAGCGTGATTGGTTTCTA	GAGTCTCTGCACCTATCCCT	50.0	55.0	58.0	57.9	1596-1764 bp	1.0	91.3
trnT-TGT-trnF-GAA_p2	CGGAGCGTGATTGGTTTCTA	GTTTGTTCGCGTAAACCAGG	50.0	50.0	58.0	58.0	1722 bp	0.5	59.7
trnT-TGT-trnF-GAA_p3	TTTTCCGGCGATACGAACAT	GTTTGTTCGCGTAAACCAGG	45.0	50.0	58.0	58.0	1829 bp	0.5	59.7
trnT-TGT-trnF-GAA_p4	CGGAGCGTGATTGGTTTCTA	TTTGTTCGCGTAAACCAGGA	50.0	45.0	58.0	58.0	1721 bp	0.5	59.6
trnT-TGT-trnF-GAA_p5	TTTTCCGGCGATACGAACAT	TTTGTTCGCGTAAACCAGGA	45.0	45.0	58.0	58.0	1828 bp	0.5	59.5