Hotspot

psaA-ycf3

LSC · 41495-42246 · 752 bp

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Pi 0.0567

MarkerSeek score 79.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 40
pi: 0.0567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCATCAGCATGTAGGTTCCA	ATTGGTTGACGATCACGAGG	50.0	50.0	58.0	58.0	896-942 bp	1.0	87.1
psaA-ycf3_p2	ATGCCACTCAGCCAAAGAAA	ATTGGTTGACGATCACGAGG	45.0	50.0	58.0	58.0	1001-1047 bp	1.0	86.8
psaA-ycf3_p3	GATCACTCAGCCATGCTTCA	ATTGGTTGACGATCACGAGG	50.0	50.0	58.0	58.0	1051-1097 bp	1.0	86.2
psaA-ycf3_p4	ATGCCACTCAGCCAAAGAAA	TTGAGCAGCGGTGTAGAATC	45.0	50.0	58.0	58.0	1639 bp	0.5	59.9
psaA-ycf3_p5	GATCACTCAGCCATGCTTCA	TTGAGCAGCGGTGTAGAATC	50.0	50.0	58.0	58.0	1689 bp	0.5	59.6