Hotspot

trnT-GGT-psbD

LSC · 31263-32417 · 1155 bp

Back to Thlaspi

Pi 0.0641

MarkerSeek score 84.40

Primer available yes

Label trnT-GGT-psbD

Feature Metadata

label_name: trnT-GGT-psbD
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 70
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGT-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1309-1393 bp	1.0	87.1
trnT-GGT-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1312-1396 bp	1.0	86.9
trnT-GGT-psbD_p3	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1326-1410 bp	1.0	86.6
trnT-GGT-psbD_p4	GACGGCCCTTTTAACTCAGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.8	58.0	1327-1411 bp	1.0	86.0
trnT-GGT-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.3	58.0	1286-1370 bp	1.0	85.5