Hotspot

trnD-GTC-trnY-GTA

LSC · 29767-30206 · 440 bp

Back to Thlaspi

Pi 0.0856

MarkerSeek score 79.70

Primer available yes

Label trnD-GTC-trnY-GTA

Feature Metadata

label_name: trnD-GTC-trnY-GTA
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 32
pi: 0.0856
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-trnY-GTA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	446-510 bp	1.0	83.6
trnD-GTC-trnY-GTA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	445-509 bp	1.0	83.6
trnD-GTC-trnY-GTA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	467-531 bp	1.0	82.5
trnD-GTC-trnY-GTA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	466-530 bp	1.0	82.3
trnD-GTC-trnY-GTA_p5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	500-564 bp	1.0	81.7