Hotspot

trnC-GCA-petN

LSC · 27347-27981 · 635 bp

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Pi 0.0718

MarkerSeek score 82.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 44
pi: 0.0718
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCATGCGAGACTTACTA	50.0	50.0	58.1	58.2	679-691 bp	1.0	85.3
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCATGCGAGACTTACTA	50.0	50.0	58.4	58.2	697-709 bp	1.0	82.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	ATTAAACCAGCCCATGCGAG	50.0	50.0	58.1	58.6	687-699 bp	1.0	82.1
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ACCATTAAACCAGCCCATGC	50.0	50.0	58.1	58.8	690-702 bp	1.0	80.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCCCATGCGAGACTTACTAT	50.0	50.0	58.1	57.2	678-690 bp	1.0	80.5