Hotspot

trnR-TCT-atpA

LSC · 9423-9802 · 380 bp

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Pi 0.13

MarkerSeek score 74.40

Primer available yes

Label trnR-TCT-atpA

Feature Metadata

label_name: trnR-TCT-atpA
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 38
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-atpA_p1	AGCCTTCCAAGCTAACGATG	ACGAATAAACCGCAGTTCCA	50.0	45.0	58.0	57.8	706-773 bp	1.0	89.3
trnR-TCT-atpA_p2	ACCCTTAGCCTTCCAAGCTA	ACGAATAAACCGCAGTTCCA	50.0	45.0	58.0	57.8	712-779 bp	1.0	89.2
trnR-TCT-atpA_p3	AGCCTTCCAAGCTAACGATG	GAGACCCAGTCAGGAGATGT	50.0	55.0	58.0	58.1	1144-1211 bp	1.0	87.3
trnR-TCT-atpA_p4	ACCCTTAGCCTTCCAAGCTA	GAGACCCAGTCAGGAGATGT	50.0	55.0	58.0	58.1	1150-1217 bp	1.0	87.3
trnR-TCT-atpA_p5	AAACCCTTAGCCTTCCAAGC	GAGACCCAGTCAGGAGATGT	50.0	55.0	58.1	58.1	1152-1219 bp	1.0	86.9