Hotspot

trnS-GCT-trnT-CGT

LSC · 7816-8410 · 595 bp

Back to Thlaspi

Pi 0.11

MarkerSeek score 84.60

Primer available yes

Label trnS-GCT-trnT-CGT

Feature Metadata

label_name: trnS-GCT-trnT-CGT
feature_type: igs
region: LSC
length_bp: 595
variable_sites: 63
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnT-CGT_p1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	660-748 bp	1.0	59.8
trnS-GCT-trnT-CGT_p2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	658-746 bp	1.0	59.5
trnS-GCT-trnT-CGT_p3	CCAACGCTTTAGTCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	57.8	57.3	662-750 bp	1.0	58.7
trnS-GCT-trnT-CGT_p4	AACGCTTTAGTCCACTCAGC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	660-748 bp	1.0	58.5
trnS-GCT-trnT-CGT_p5	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	50.0	36.4	57.8	56.2	661-749 bp	1.0	57.9