Hotspot

rps16-trnQ-TTG

LSC · 6098-6553 · 456 bp

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Pi 0.124
MarkerSeek score 84.90
Primer available yes
Label rps16-trnQ-TTG

Feature Metadata

label_name
rps16-trnQ-TTG
feature_type
igs
region
LSC
length_bp
456
variable_sites
53
pi
0.124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-TTG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 517-529 bp 1.0 90.3
rps16-trnQ-TTG_p2 CCTTCAATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 527-539 bp 1.0 89.5
rps16-trnQ-TTG_p3 TCAATTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 524-536 bp 1.0 86.1
rps16-trnQ-TTG_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 521-533 bp 1.0 85.5
rps16-trnQ-TTG_p5 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 547-559 bp 1.0 85.1