Hotspot

ycf1

SSC · 126801-132338 · 5538 bp

Back to Thladiantha

Pi 0.213

MarkerSeek score 75.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 1767
pi: 0.213
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCCCGTAACCAGTTTTTCC	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	58.0	58.0	1053-1059 bp	0.5	61.4
ycf1_p2	CCCCGTAACCAGTTTTTCCA	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	58.0	58.0	1052-1058 bp	0.5	61.4
ycf1_p3	ACCCCGTAACCAGTTTTTCC	GGTTCTCCCCGAAAACAACT	50.0	50.0	58.0	58.0	1047-1053 bp	0.5	61.3
ycf1_p4	ATGCTTGCGACAAGTACGAT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	58.0	58.0	841 bp	0.5	60.9
ycf1_p5	ATGCTTGCGACAAGTACGAT	GGTTCTCCCCGAAAACAACT	45.0	50.0	58.0	58.0	835 bp	0.5	60.8