Hotspot

ndhI

SSC · 122033-122527 · 495 bp

Back to Thladiantha

Pi 0.284

MarkerSeek score 79.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 495
variable_sites: 212
pi: 0.284
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CATGTATCGGTCCAGGCAAA	AAATAATCCCGACACGCTCC	50.0	50.0	58.0	58.1	1618-1620 bp	0.5	60.0
ndhI_p2	CAAGAACCAGTCCCAACGAA	AAATAATCCCGACACGCTCC	50.0	50.0	58.0	58.1	1723-1725 bp	0.5	60.0
ndhI_p3	CGGTCCAGGCAAATCCATTA	AAATAATCCCGACACGCTCC	50.0	50.0	57.9	58.1	1611-1613 bp	0.5	59.9
ndhI_p4	CATGTATCGGTCCAGGCAAA	TGGTTTTTGGGGGTGGAATT	50.0	45.0	58.0	57.8	1449-1451 bp	0.5	58.9
ndhI_p5	CAAGAACCAGTCCCAACGAA	TGGTTTTTGGGGGTGGAATT	50.0	45.0	58.0	57.8	1554-1556 bp	0.5	58.9