Hotspot

ccsA

SSC · 117247-118218 · 972 bp

Back to Thladiantha

Pi 0.25

MarkerSeek score 79.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 364
pi: 0.25
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TTATCGATGGCTTCCCTTGC	50.0	50.0	58.0	58.0	1758-1767 bp	0.5	61.5
ccsA_p2	TGAAATCGGTAGACACGCTG	TTATCGATGGCTTCCCTTGC	50.0	50.0	58.0	58.0	1757-1766 bp	0.5	61.5
ccsA_p3	GAGAAAGCCGCTATGGTGAA	TTATCGATGGCTTCCCTTGC	50.0	50.0	58.0	58.0	1773-1782 bp	0.5	61.4
ccsA_p4	GTGAAATCGGTAGACACGCT	CATGTTTGTGTTCGTCAGGC	50.0	50.0	58.0	58.0	1177 bp	0.5	60.7
ccsA_p5	TGAAATCGGTAGACACGCTG	CATGTTTGTGTTCGTCAGGC	50.0	50.0	58.0	58.0	1176 bp	0.5	60.7