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psaC-ndhE

SSC · 120367-120613 · 247 bp

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Pi 0.282

MarkerSeek score 76.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 247
variable_sites: 105
pi: 0.282
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGAAAAGTAAGCAACCCGCA	TCTTAGCTGCTTTGATCGGC	45.0	50.0	58.0	58.3	1276-1282 bp	0.5	58.2
psaC-ndhE_p2	AGAAAAGTAAGCAACCCGCA	GGATTGATCACGAGCCGAAA	45.0	50.0	58.0	58.3	954-960 bp	0.5	57.9
psaC-ndhE_p3	GCTTCGAGTTGTTTCATGCC	TCTTAGCTGCTTTGATCGGC	50.0	50.0	58.0	58.3	1046-1047 bp	0.5	57.8
psaC-ndhE_p4	ACCCATGCTTCGAGTTGTTT	TCTTAGCTGCTTTGATCGGC	45.0	50.0	58.0	58.3	1052 bp	0.25	47.1
psaC-ndhE_p5	ACCCATGCTTCGAGTTGTTT	GGATTGATCACGAGCCGAAA	45.0	50.0	58.0	58.3	730 bp	0.25	47.1