Hotspot

trnL-UAG-ccsA

SSC · 117149-117246 · 98 bp

Back to Thladiantha

Pi 0.26

MarkerSeek score 77.40

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 98
variable_sites: 39
pi: 0.26
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	AAAATAGTACTTGGCGCGGT	50.0	45.0	58.0	57.9	498 bp	0.5	60.1
trnL-UAG-ccsA_p2	GTGAAATCGGTAGACACGCT	AAATAGTACTTGGCGCGGTT	50.0	45.0	58.0	57.9	497 bp	0.5	60.1
trnL-UAG-ccsA_p3	TGAAATCGGTAGACACGCTG	AAAATAGTACTTGGCGCGGT	50.0	45.0	58.0	57.9	497 bp	0.5	60.1
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	AAATAGTACTTGGCGCGGTT	50.0	45.0	58.0	57.9	496 bp	0.5	60.1
trnL-UAG-ccsA_p5	GAGAAAGCCGCTATGGTGAA	AAATAGTACTTGGCGCGGTT	50.0	45.0	58.0	57.9	512 bp	0.5	59.9