Hotspot

rpl32-trnL-UAG

SSC · 115992-117068 · 1077 bp

Back to Thladiantha

Pi 0.266

MarkerSeek score 79.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1077
variable_sites: 428
pi: 0.266
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	1166-1191 bp	0.5	60.6
rpl32-trnL-UAG_p2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	1167-1192 bp	0.5	60.6
rpl32-trnL-UAG_p3	GGCAGCGTTGAAAGCTTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.4	57.9	1176-1201 bp	0.5	59.7
rpl32-trnL-UAG_p4	GGCAGCGTTGAAAGCTTTTT	AAAATAGTACTTGGCGCGGT	45.0	45.0	58.4	57.9	1644-1669 bp	0.5	59.0
rpl32-trnL-UAG_p5	GGCAGCGTTGAAAGCTTTTT	AAATAGTACTTGGCGCGGTT	45.0	45.0	58.4	57.9	1643-1668 bp	0.5	59.0