Hotspot

ndhF-rpl32

SSC · 115177-115829 · 653 bp

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Pi 0.286

MarkerSeek score 78.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 653
variable_sites: 260
pi: 0.286
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	AAAAAGCTTTCAACGCTGCC	45.0	45.0	58.0	58.4	738-849 bp	0.5	64.2
ndhF-rpl32_p2	CCCCACGCGATTAGTTACAA	AAAAAGCTTTCAACGCTGCC	50.0	45.0	58.0	58.4	1194-1305 bp	0.5	63.3
ndhF-rpl32_p3	CCCCACGCGATTAGTTACAA	AGTCATCCGCTCAAAACAGT	50.0	45.0	58.0	57.4	1479-1608 bp	0.5	62.0
ndhF-rpl32_p4	CCCCACGCGATTAGTTACAA	TGTTTTATTGGGGCGATGGG	50.0	50.0	58.0	58.5	1815 bp	0.25	45.7
ndhF-rpl32_p5	TGTTGCCGTCGGAAAAAGTA	TGTTTTATTGGGGCGATGGG	45.0	50.0	58.0	58.5	1359 bp	0.25	45.4