Hotspot

trnM-CAU-atpE

LSC · 55695-55859 · 165 bp

Back to Thladiantha

Pi 0.065

MarkerSeek score 81.10

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 18
pi: 0.065
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	CGATGTGATAGGGCCCATTT	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.7	58.0	334-356 bp	1.0	82.0
trnM-CAU-atpE_p2	CGATGTGATAGGGCCCATTT	CGAGCTAGGACACGAGTAGA	50.0	55.0	57.7	58.1	326-348 bp	1.0	81.8
trnM-CAU-atpE_p3	CGATGTGATAGGGCCCATTT	AAAGCTCAGGGCAAAAGACA	50.0	45.0	57.7	57.9	374-396 bp	1.0	81.2
trnM-CAU-atpE_p4	CGATGTGATAGGGCCCATTT	AAGCTCAGGGCAAAAGACAA	50.0	45.0	57.7	57.9	373-395 bp	1.0	81.2
trnM-CAU-atpE_p5	CGATGTGATAGGGCCCATTT	AGCTCAGGGCAAAAGACAAA	50.0	45.0	57.7	57.9	372-394 bp	1.0	81.2