Hotspot

ndhJ-ndhK

LSC · 52777-52889 · 113 bp

Back to Thladiantha

Pi 0.059

MarkerSeek score 80.30

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 11
pi: 0.059
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GAAGACAAACGACCCTGCAT	ACAAAAGTTCAGTATCTTCCTACGA	50.0	36.0	58.5	58.0	163-218 bp	1.0	48.9
ndhJ-ndhK_p2	CCAAGAAGACAAACGACCCT	ACAAAAGTTCAGTATCTTCCTACGA	50.0	36.0	57.7	58.0	167-222 bp	1.0	48.8
ndhJ-ndhK_p3	AAGAAGACAAACGACCCTGC	ACAAAAGTTCAGTATCTTCCTACGA	50.0	36.0	58.4	58.0	165-220 bp	1.0	48.8
ndhJ-ndhK_p4	CCAAGAAGACAAACGACCCT	ACAAAAGTTCAGTATCTTCCTACG	50.0	37.5	57.7	56.7	167-222 bp	1.0	48.8
ndhJ-ndhK_p5	AGCCAAGAAGACAAACGACC	ACAAAAGTTCAGTATCTTCCTACGA	50.0	36.0	58.4	58.0	169-224 bp	1.0	48.7