Hotspot

psbC

LSC · 9598-11019 · 1422 bp

Back to Thinopyrum

Pi 0.00141

MarkerSeek score 48.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 5
pi: 0.00141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TGTTTCCCAGGAAATCCGTG	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.1	58.0	1603 bp	1.0	79.4
psbC_p2	GCAGCGGAAGATCCTGAATT	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.3	58.0	1584 bp	1.0	77.6
psbC_p3	TGTTTCCCAGGAAATCCGTG	AACCGAGCCAGAACAGAAAA	50.0	45.0	58.1	57.7	1584 bp	1.0	76.8
psbC_p4	TGTTTCCCAGGAAATCCGTG	ACCGAGCCAGAACAGAAAAA	50.0	45.0	58.1	57.7	1583 bp	1.0	76.8
psbC_p5	TGTTTCCCAGGAAATCCGTG	GGAATAACCGAGCCAGAACA	50.0	50.0	58.1	57.6	1589 bp	1.0	76.3