Hotspot

ndhF

SSC · 101506-103725 · 2220 bp

Back to Thinopyrum

Pi 0.00171

MarkerSeek score 37.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 9
pi: 0.00171
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	GTATGGAAGCCTATCCCCCA	45.0	55.0	58.0	58.3	2952-2960 bp	1.0	78.2
ndhF_p2	AAAATCGCGGAAAACCGAAC	TGGTATGGAAGCCTATCCCC	45.0	55.0	58.0	58.3	2954-2962 bp	1.0	78.2
ndhF_p3	AATCGCGGAAAACCGAACTA	GTATGGAAGCCTATCCCCCA	45.0	55.0	57.9	58.3	2950-2958 bp	1.0	77.9
ndhF_p4	TCGCGGAAAACCGAACTATT	GTATGGAAGCCTATCCCCCA	45.0	55.0	57.9	58.3	2948-2956 bp	1.0	77.9
ndhF_p5	TCGCGGAAAACCGAACTATT	TGGTATGGAAGCCTATCCCC	45.0	55.0	57.9	58.3	2950-2958 bp	1.0	77.9