Hotspot

ndhA

SSC · 111100-113214 · 2115 bp

Back to Thinopyrum

Pi 0.0017

MarkerSeek score 58.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2115
variable_sites: 8
pi: 0.0017
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.1	57.9	2256 bp	1.0	70.6
ndhA_p2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.1	57.8	2254 bp	1.0	70.1
ndhA_p3	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2255 bp	1.0	65.0
ndhA_p4	ACCCAGTTACCATAGGGAAC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2256 bp	1.0	64.9
ndhA_p5	CCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	50.0	50.0	56.2	57.8	2253 bp	1.0	64.4