Hotspot

psaC-ndhE

SSC · 108674-109144 · 471 bp

Back to Thinopyrum

Pi 0.00594

MarkerSeek score 40.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 471
variable_sites: 6
pi: 0.00594
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCTTTACATCCATCCCAGGG	ACTCGTATCAATCAATCTAATTTATTG	55.0	25.9	58.0	54.6	614-620 bp	1.0	40.8
psaC-ndhE_p2	GGCTTTACATCCATCCCAGG	ACTCGTATCAATCAATCTAATTTATTG	55.0	25.9	58.0	54.6	615-621 bp	1.0	40.8
psaC-ndhE_p3	GTTGGACAAGCTCGTACACA	ACTCGTATCAATCAATCTAATTTATTG	50.0	25.9	58.1	54.6	575-581 bp	1.0	40.8
psaC-ndhE_p4	TGTTGGACAAGCTCGTACAC	ACTCGTATCAATCAATCTAATTTATTG	50.0	25.9	58.1	54.6	576-582 bp	1.0	40.8
psaC-ndhE_p5	CTGTTGGACAAGCTCGTACA	ACTCGTATCAATCAATCTAATTTATTG	50.0	25.9	57.9	54.6	577-583 bp	1.0	40.8