Hotspot

rps8-rpl14

LSC · 77136-77280 · 145 bp

Back to Thinopyrum

Pi 0.00556

MarkerSeek score 58.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 2
pi: 0.00556
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTGTCCTTGCC	CGATTGCCGAGGAATTGAGA	50.0	50.0	57.9	58.1	233-236 bp	1.0	80.3
rps8-rpl14_p2	GCAATAGTGTCCTTGCCCAT	CGATTGCCGAGGAATTGAGA	50.0	50.0	58.2	58.1	230-233 bp	1.0	79.4
rps8-rpl14_p3	TCAGCAATAGTGTCCTTGCC	GGCAATCCAAAAGGAACTCG	50.0	50.0	57.9	57.4	264-267 bp	1.0	75.5
rps8-rpl14_p4	GCAATAGTGTCCTTGCCCAT	GGCAATCCAAAAGGAACTCG	50.0	50.0	58.2	57.4	261-264 bp	1.0	74.6
rps8-rpl14_p5	TCAGCAATAGTGTCCTTGCC	AGGAACTCGAGTTTTTGGCG	50.0	50.0	57.9	58.8	253-256 bp	1.0	74.5