Hotspot

psaA-ycf3

LSC · 41120-41824 · 705 bp

Back to Thinopyrum

Pi 0

MarkerSeek score 64.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 0
pi: 0
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	835-866 bp	1.0	82.4
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	831-862 bp	1.0	82.0
psaA-ycf3_p3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	833-864 bp	1.0	81.2
psaA-ycf3_p4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	834-865 bp	1.0	81.2
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	50.0	50.0	57.8	58.1	829-860 bp	1.0	80.9