Hotspot

trnC-GCA-rpoB

LSC · 18149-19307 · 1159 bp

Back to Thinopyrum

Pi 0.0019

MarkerSeek score 55.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 5
pi: 0.0019
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1223-1228 bp	1.0	79.8
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1230-1235 bp	1.0	78.7
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1238-1243 bp	1.0	77.5
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1237-1242 bp	1.0	77.5
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	50.0	45.0	58.1	57.6	1302-1307 bp	1.0	77.1