Hotspot

psbM-petN

LSC · 16788-17526 · 739 bp

Back to Thinopyrum

Pi 0.00379

MarkerSeek score 65.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 6
pi: 0.00379
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	GTATGGGGAAGGAGTGGACT	42.9	55.0	57.6	58.1	828-842 bp	1.0	70.7
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	AGTATGGGGAAGGAGTGGAC	42.9	55.0	57.6	58.1	829-843 bp	1.0	70.6
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	57.6	58.2	887-901 bp	1.0	70.2
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	TATGGGGAAGGAGTGGACTC	42.9	55.0	57.6	57.8	827-841 bp	1.0	70.2
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	GGGGAAGGAGTGGACTCTAG	42.9	60.0	57.6	58.2	824-838 bp	1.0	69.9