Hotspot

trnY-GUA-trnD-GUC

LSC · 15452-15810 · 359 bp

Back to Thinopyrum

Pi 0.00353

MarkerSeek score 56.70

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 359
variable_sites: 2
pi: 0.00353
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	479-483 bp	1.0	76.5
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	446-450 bp	1.0	74.1
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	445-449 bp	1.0	73.9
trnY-GUA-trnD-GUC_p4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	50.0	50.0	58.0	57.0	478-482 bp	1.0	72.3
trnY-GUA-trnD-GUC_p5	GATGCCCGAGTGGTTAATGG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.7	58.5	464-468 bp	1.0	71.3