Hotspot

lhbA-trnG-UCC

LSC · 11798-12074 · 277 bp

Back to Thinopyrum

Pi 0.00505

MarkerSeek score 26.40

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 3
pi: 0.00505
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	TATTCCATCTAGCCGAGCCA	AACAGTTTGCCCGTTGTTTG	50.0	45.0	58.0	58.0	1309-1311 bp	1.0	80.4
lhbA-trnG-UCC_p2	CAACCACTCAGCCATCTCTC	AACAGTTTGCCCGTTGTTTG	55.0	45.0	58.0	58.0	1146 bp	1.0	80.4
lhbA-trnG-UCC_p3	TATTCCATCTAGCCGAGCCA	TGGCGAGTTCTATTTCTGCC	50.0	50.0	58.0	58.0	1257-1259 bp	1.0	80.2
lhbA-trnG-UCC_p4	CAACCACTCAGCCATCTCTC	TGGCGAGTTCTATTTCTGCC	55.0	50.0	58.0	58.0	1094 bp	1.0	80.2
lhbA-trnG-UCC_p5	TATTCCATCTAGCCGAGCCA	AGCCTTCCAAGCTAACGATG	50.0	50.0	58.0	58.0	1735-1737 bp	1.0	80.1