Hotspot

trnI-GAU

IRa · 141755-141796 · 42 bp

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Pi 0.0238
MarkerSeek score 66.20
Primer available yes
Label trnI-GAU

Feature Metadata

label_name
trnI-GAU
feature_type
tRNA
region
IRa
length_bp
42
variable_sites
1
pi
0.0238
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU_p1 GGGGGCGTAAAAAGGAAAGA CTATCCATGTGGCTGAGAGC 50.0 55.0 58.1 57.8 164 bp 1.0 78.5
trnI-GAU_p2 GGGGGCGTAAAAAGGAAAGA CGCTGATGAGCACATTGAAC 50.0 50.0 58.1 57.8 183 bp 1.0 78.4
trnI-GAU_p3 AGAGAGGGATGGGGTTTCTC CTATCCATGTGGCTGAGAGC 55.0 55.0 58.1 57.8 147 bp 1.0 78.3
trnI-GAU_p4 AGAGGGATGGGGTTTCTCTC CTATCCATGTGGCTGAGAGC 55.0 55.0 58.1 57.8 145 bp 1.0 78.3
trnI-GAU_p5 GAGAGGGATGGGGTTTCTCT CTATCCATGTGGCTGAGAGC 55.0 55.0 58.1 57.8 146 bp 1.0 78.3
trnI-GAU_p1 CTATCCATGTGGCTGAGAGC GGGGGCGTAAAAAGGAAAGA 55.0 50.0 57.8 58.1 164 bp 1.0 78.5
trnI-GAU_p2 CGCTGATGAGCACATTGAAC GGGGGCGTAAAAAGGAAAGA 50.0 50.0 57.8 58.1 183 bp 1.0 78.4
trnI-GAU_p3 CTATCCATGTGGCTGAGAGC AGAGAGGGATGGGGTTTCTC 55.0 55.0 57.8 58.1 147 bp 1.0 78.3
trnI-GAU_p4 CTATCCATGTGGCTGAGAGC AGAGGGATGGGGTTTCTCTC 55.0 55.0 57.8 58.1 145 bp 1.0 78.3
trnI-GAU_p5 CTATCCATGTGGCTGAGAGC GAGAGGGATGGGGTTTCTCT 55.0 55.0 57.8 58.1 146 bp 1.0 78.3