Hotspot

ndhF-trnN-GUU

IRa · 134686-135248 · 563 bp

Back to Thespesia

Pi 0.04

MarkerSeek score 71.00

Primer available yes

Label ndhF-trnN-GUU

Feature Metadata

label_name: ndhF-trnN-GUU
feature_type: igs
region: IRa
length_bp: 563
variable_sites: 20
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnN-GUU_p1	TGAATAACAACGAGCAGCCA	GGGTTACCTGCTCTTAACGG	45.0	55.0	57.8	58.0	1351-1420 bp	1.0	83.8
ndhF-trnN-GUU_p2	TGAATAACAACGAGCAGCCA	ACGACCAGTCAGTTAACAGC	45.0	50.0	57.8	57.8	1250-1319 bp	1.0	82.9
ndhF-trnN-GUU_p3	TGAATAACAACGAGCAGCCA	GGCAGAATAGCAGAGCAAGT	45.0	50.0	57.8	58.0	1659-1723 bp	1.0	82.7
ndhF-trnN-GUU_p4	TGAATAACAACGAGCAGCCA	TAATGTGTTTGCTCGCGGTA	45.0	45.0	57.8	57.9	1594-1658 bp	1.0	82.6
ndhF-trnN-GUU_p5	TGAATAACAACGAGCAGCCA	ATCATGATCGGGATAGCGGA	45.0	50.0	57.8	58.1	1852-1917 bp	1.0	81.6