Hotspot

rps19-rpl2

IRb · 88971-89047 · 77 bp

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Pi 0.117

MarkerSeek score 87.50

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 77
variable_sites: 9
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGCTCTGGACCAA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	280-289 bp	1.0	80.4
rps19-rpl2_p2	ATGGTAGATGCTCTGGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.8	57.8	281-290 bp	1.0	79.4
rps19-rpl2_p3	TGGTAGATGCTCTGGACCAA	CTTCGTCGCCGGAGTAAATA	50.0	50.0	58.0	57.5	216-225 bp	1.0	79.0
rps19-rpl2_p4	TGGTAGATGCTCTGGACCAA	TTCGTCGCCGGAGTAAATAG	50.0	50.0	58.0	57.5	215-224 bp	1.0	79.0
rps19-rpl2_p5	TGGTAGATGCTCTGGACCAA	GGGTTATCCTGCACTTGGAA	50.0	50.0	58.0	57.5	277-286 bp	1.0	78.1