Hotspot

ndhC-trnV-UAC

LSC · 53637-54779 · 1143 bp

Back to Thespesia

Pi 0.0239

MarkerSeek score 66.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 27
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATCGGGGCTA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	57.8	57.9	1345-1374 bp	1.0	80.0
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	57.8	57.9	1333-1362 bp	1.0	80.0
ndhC-trnV-UAC_p3	ACCCTTTCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1287-1316 bp	1.0	79.5
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1275-1304 bp	1.0	79.5
ndhC-trnV-UAC_p5	ATCGGGGCTAAAACTCCAGA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	58.4	57.9	1335-1364 bp	1.0	78.1