Hotspot

rps4-trnT-UGU

LSC · 48162-48701 · 540 bp

Back to Thespesia

Pi 0.0321

MarkerSeek score 69.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 540
variable_sites: 17
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	689-698 bp	1.0	82.0
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	693-702 bp	1.0	81.6
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	657-666 bp	1.0	81.3
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	661-670 bp	1.0	80.9
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	697-706 bp	1.0	79.0