Hotspot

psbZ-trnG-GCC

LSC · 37779-38542 · 764 bp

Back to Thespesia

Pi 0.0413

MarkerSeek score 87.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 30
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	921-939 bp	1.0	82.9
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	919-937 bp	1.0	82.9
psbZ-trnG-GCC_p3	CTCCTGATGGTTGGTTGAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	915-933 bp	1.0	77.6
psbZ-trnG-GCC_p4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	920-938 bp	1.0	77.0
psbZ-trnG-GCC_p5	TCTCCTGATGGTTGGTTGAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	916-934 bp	1.0	75.3