Hotspot

trnH-psbA

LSC · 76-421 · 346 bp

Back to Thespesia

Pi 0.0557

MarkerSeek score 74.80

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 19
pi: 0.0557
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	416-439 bp	1.0	82.9
trnH-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	430-453 bp	1.0	82.7
trnH-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	429-452 bp	1.0	82.7
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	431-454 bp	1.0	82.7
trnH-psbA_p5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	465-488 bp	1.0	80.3