Hotspot

rps15-ycf1

SSC · 107686-108024 · 339 bp

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Pi 0.0669

MarkerSeek score 77.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 339
variable_sites: 42
pi: 0.0669
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGAAATTCGACTGATCCCCG	TTCTTTGGCCCAATTATCGA	50.0	40.0	57.7	55.0	538-561 bp	1.0	62.1
rps15-ycf1_p2	TGAAATTCGACTGATCCCCG	TCGAACCAATTTGTTTGTCTGT	50.0	36.4	57.7	57.0	907-916 bp	0.75	46.9
rps15-ycf1_p3	TGAAATTCGACTGATCCCCG	CAGCAAATGAATCAAAGCTACCA	50.0	39.1	57.7	57.9	821-824 bp	0.75	46.2
rps15-ycf1_p4	TGAAATTCGACTGATCCCCG	ACAGCAAATGAATCAAAGCTACC	50.0	39.1	57.7	57.9	822-825 bp	0.75	46.2
rps15-ycf1_p5	GAAATTCGACTGATCCCCGG	TCGAACCAATTTGTTTGTCTGT	55.0	36.4	58.4	57.0	906-915 bp	0.75	46.0