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psaC-rps15

SSC · 106780-107412 · 633 bp

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Pi 0.0572

MarkerSeek score 86.10

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 633
variable_sites: 64
pi: 0.0572
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	CATAAATCTTTACTGAATGTGACA	TCAGTTGAATATTCGGGAAC	29.2	40.0	53.4	53.0	681-723 bp	1.0	51.4
psaC-rps15_p2	TCATAAATCTTTACTGAATGTGACA	TCAGTTGAATATTCGGGAAC	28.0	40.0	54.8	53.0	682-724 bp	1.0	51.3
psaC-rps15_p3	ATCATAAATCTTTACTGAATGTGACA	TCAGTTGAATATTCGGGAAC	26.9	40.0	55.3	53.0	683-725 bp	1.0	51.3
psaC-rps15_p4	TCATAAATCTTTACTGAATGTGACAT	TCAGTTGAATATTCGGGAAC	26.9	40.0	55.3	53.0	682-724 bp	1.0	51.3
psaC-rps15_p5	TCATAAATCTTTACTGAATGTGAC	TCAGTTGAATATTCGGGAAC	29.2	40.0	53.2	53.0	682-724 bp	1.0	51.3