Hotspot

rpl16-rps3

LSC · 78758-78938 · 181 bp

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Pi 0.0589
MarkerSeek score 80.70
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
181
variable_sites
21
pi
0.0589
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AACGAGTCACACACTAAGCA TGCTCCTATCCAATTCGAACT 45.0 42.9 57.1 56.8 268-283 bp 1.0 63.3
rpl16-rps3_p2 ACGAGTCACACACTAAGCAT TGCTCCTATCCAATTCGAACT 45.0 42.9 56.9 56.8 267-282 bp 1.0 61.5
rpl16-rps3_p3 AACGAGTCACACACTAAGCA TTGCTCCTATCCAATTCGAACT 45.0 40.9 57.1 57.5 269-284 bp 1.0 60.9
rpl16-rps3_p4 AACGAGTCACACACTAAGCAT TGCTCCTATCCAATTCGAACT 42.9 42.9 57.6 56.8 268-283 bp 1.0 59.2
rpl16-rps3_p5 ACGAGTCACACACTAAGCAT TTGCTCCTATCCAATTCGAACT 45.0 40.9 56.9 57.5 268-283 bp 1.0 59.1